JIB-04
作者：齊岳
發(fā)布時(shí)間：2023-09-06
08:59:45
點(diǎn)擊數(shù)：
產(chǎn)品名稱：JIB-04
產(chǎn)品描述：
| 產(chǎn)品描述 | JIB-04 is a pan-selective Jumonji histone demethylase inhibitor. |
| 靶點(diǎn)活性 | JARID1A:230 nM, JMJD2B:435 nM, JMJD2E:340 nM, JMJD2D:290 nM, JMJD2A:445 nM |
| 體外活性 | 在H358和A549小鼠異種移植模型中,JIB-04抑制Jumonji組蛋白脫甲基酶的活性,抑制腫瘤細(xì)胞生長(zhǎng). |
| 體內(nèi)活性 | 在肺癌和前列腺癌細(xì)胞系中（IC50=10 nM）,JIB-04下調(diào)增殖基因,阻斷細(xì)胞生長(zhǎng)。 |
| 細(xì)胞實(shí)驗(yàn) | For cell viability assays, cells are plated at 1500-3000 cells/well in 96 well plates and treated the next day with increasing doses of compound over 4 days and their viability assessed by standard MTS assays using Promega’s Cell Titer or Cell Titer-Glo reagents according to the manufacturer’s protocols. Absorbance at 490 nm and 650 nm or luminescence is measured by a Spectra Max or a FlouroStar Omega plate reader. Data are normalized to the untreated controls (100% viability). Each cell line is tested in 2-5 independent assays, each containing 4-8 replicates. IC50 values are calculated using DIVISA, a high-throughput software, developed in hous, for storing and analyzing drug sensitivity assays. Dose-response curves are plotted using a non-linear regression model and IC50s are determined from the fitted curves. The average IC50 derived from 2-5 independent assays, each containing 4-8 replicates is reported. (Only for Reference) |
| 別名 | NSC 693627, 5-氯-2(1H)-吡啶酮 (2E)-(苯基-2-吡啶基亞甲基)腙, JIB 04 |
| 分子量 | 308.76 |
| 分子式 | C17H13ClN4 |
| CAS No. | 199596-05-9 |
存儲(chǔ)
Powder: -20°C for 3 years | In solvent: -80°C for 2 years
溶解度
DMSO: 30.9 mg/mL (100 mM)
( < 1 mg/mL refers to the product slightly soluble or insoluble )
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